Functional analysis of IP3 receptors

I developed a high-throughput fluorescence polarisation (FP) binding assay using fluorescein-IP<sub>3</sub> and purified N-terminal fragments of IP<sub>3</sub>R to examine the thermodynamics of ligand binding to IP<sub>3</sub>R. I demonstrate that at 4°C, equilibr...

Full description

Bibliographic Details
Main Author: Ding, Z.
Published: University of Cambridge 2010
Subjects:
572
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.598546