Biophysical studies of the green fluorescent protein

Chemical denaturation was used to unfold the protein, changes in structure being monitored by the green fluorescence. The denaturation behaviour of GFP was found to be complex compared to many small proteins: equilibrium was established only very slowly, over the time course of weeks, suggesting the...

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Bibliographic Details
Main Author: Craggs, T. D.
Published: University of Cambridge 2007
Subjects:
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.598115