Target site selection of retroviral vectors in the human genome : viral and genomic determinants of non-random integration patterns in hematopoietic cells
Integration of gamma-retroviruses (RV) and lehtiviruses (LV) follows different, non random patterns in mammalian genomes. To obtain information about the viral and genomic determinants of integration preferences, I mapped > 2,500 integration sites of RV and LV vectors carrying wild type or modifi...
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Open University
2008
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.494505 |
| Summary: | Integration of gamma-retroviruses (RV) and lehtiviruses (LV) follows different, non random patterns in mammalian genomes. To obtain information about the viral and genomic determinants of integration preferences, I mapped > 2,500 integration sites of RV and LV vectors carrying wild type or modified LTRs in human CD34+ hematopoietic cells. To investigate the role of transcriptional regulatory networks in directing RV and LV integration, 1 evaluated the local abundance and anangement of putative transcription factor binding sites (TFBSs) in the genomic regions flanking integrated proviruses. |
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