Characterisacion of flavocytochrome P450 BM3 site directed mutants with novel heme ligation state
Uniquely among cytochromes P450, family 4 P450s have a conserved glutamate covalently attaching heme to the protein backbone. In the related Bacillus megaterium P450 BM3, the corresponding residue is alanine (A264). The relevant mutation (A264E) was constructed and characterised spectroscopically, k...
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University of Leicester
2005
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.422868 |