A fructose-intolerant yeast strain to select for sucrose fructosyl-transferase activity

A selection system for yeast cells expressing mutated invertase (EC 3.2.1.26, SUC2) with altered fructotransferase activity was developed based on the survival of a fructose-intolerant strain in the presence of suitable acceptor substrates and sucrose. Cells of such a strain expressing a wild-type h...

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Bibliographic Details
Main Author: Doyle, Timothy Charles
Other Authors: Donaldson, Iain
Published: University of Oxford 1993
Subjects:
572
Online Access:http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.333060