Cloning and expression of a human type V acid phosphatase single chain antibody fragment in Escherichia coli and Pichia pastoris

Cloning and expression of a human type V acid phosphatase single chain antibody fragment in Escherichia coli and Pichia pastoris

A recombinant single chain antibody (scAb) fragment specific for the isoenzyme human type V acid phosphatase has been expressed and secreted from Escherichia coli at a level of 0.02 mg/litre culture volume. Purification of the fragment using antibody immunoaffinity and metal chelate affinity chromat...

Full description

Bibliographic Details
Main Author:	Cupit, Pauline M.
Published:	University of Aberdeen 1996
Subjects:	572.8 Antibodies
Online Access:	http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320694

Similar Items

The cloning, page display and bacterial expression of single chain antibody fragments
by: Watkins, Nicholas Andrew
Published: (1996)

Cloning of Streptomyces genes in Escherichia coli
by: Lim, C. K.
Published: (1986)

Cloning of an Escherichia coli adhesin
by: Hinson, Gary
Published: (1987)

Cloning antibody fragments to the α2β1 integrin on platelets by V phage
by: Hughes, D. L.
Published: (2002)

Cloning and expression of iron superoxide dismutase in Escherichia coli and in yeast
by: Balzan, Rena
Published: (1993)

The molecular cloning of the mutT gene of Escherichia coli K-12
by: Moffat, K. G.
Published: (1986)

Cloning and analysis of the nupC gene of Escherichia coli, encoding a nucleoside permease
by: Craig, Jane E.
Published: (1993)

Cloning and analysis of the Escherichia coli K10 and K54 capsule gene clusters
by: Pearce, Rowan
Published: (1997)

Fluorescent amplified fragment length polymorphism typing of salmonella and Escherichia coli O157
by: Scott, Fiona Wendy
Published: (2001)

Molecular cloning and characterization of the gua regulatory region of Escherichia coli K-12
by: Thomas, M. S.
Published: (1984)

Studies on some arginine biosynthetic genes of Bacillus subtilis cloned in Escherichia coli
by: Munton, R. N.
Published: (1983)

Cloning and expression of mycobacterial genes in Escherichia coli
by: Moss, Michael T.
Published: (1987)

Illegitimate recombination in Escherichia coli
by: Blake, Catherine E.
Published: (1996)

The production of single chain antibody Fv fragments by phage display and from hybridoma genes
by: Beavis, Juliet Clare
Published: (1996)

S. pombe artificial chromosome (SPARC) vectors for cloning large DNA fragments
by: Young, Dorothy J. D.
Published: (1997)

Strategies for making antibodies by phage display
by: Bonnert, Timothy Peter
Published: (1993)

Cloning, sequence determination and expression in E. coli of the gene for the human immunoglobulin epsilon chain expressed in a myeloma cell line
by: Kenten, J. H.
Published: (1983)

The cloning and identification of the gene products of the mutH gene of Escherichia coli K-12
by: Hussain, K.
Published: (1984)

The cloning and expression of amylolytic genes in Escherichia coli and their role in starch utilization
by: Taylor, P. P.
Published: (1986)

Molecular cloning and analysis of the ruv gene of Escherichia coli K12
by: Benson, Fiona Elizabeth
Published: (1988)

Type I restriction systems of Escherichia coli
by: Barcus, Victoria Anne
Published: (1993)

CTG trinucleotide repeat instability in Escherichia coli
by: Schmidt, Kristina H.
Published: (1999)

A study of the Escherichia coli cell cycle
by: Liu, Guowen
Published: (2000)

The localization and timing of cell division in Escherichia coli
by: Teather, R. M.
Published: (1974)

Metabolic engineering of Escherichia coli for the biosynthesis of astaxanthin
by: Scaife, Mark Aden
Published: (2008)

Protein - RNA interactions in the ribosome of escherichia coli
by: Newberry, V. J.
Published: (1978)

Combinatorial antibody display libraries from sheep and their analysis
by: Charlton, Keith Alan
Published: (2000)

Plasmid vectors suitable for the expression of cloned genes under the control of the Escherichia coli Tryptophan promoter : plasmid expression vectors
by: Tacon, W. C. A.
Published: (1981)

Studies of the binding interactions of human lysozyme with camelid antibody fragments
by: Chan, P.-H.
Published: (2006)

Chromosome replication, transcription and cell division in Escherichia coli
by: Jones, Nicholas C.
Published: (1974)

Investigation of trinucleotide repeat instability in the Escherichia coli chromosome
by: Mihaescu, Camelia
Published: (2002)

The cloning and expression of the hepatitis B polymerase gene in Escherichia coli
by: McGlynn, Elaine
Published: (1987)

Cloning and analysis of genes conferring sensitivity to compound 40/80 in Escherichia coli
by: Chen, Maoxiang
Published: (1990)

The ATP-synthase of Escherichia coli
by: Gay, N. J.
Published: (1982)

Genetic mobility in Escherichia coli
by: Saul, M. W.
Published: (1981)

In vitro selection and characterisation of human anti-HIV-1 antibody fragments
by: Tang, Jie
Published: (2011)

Investigation into the role of chaperones in the secretion of haemolysin from Escherichia coli
by: Whitehead, J. P.
Published: (1994)

Genes encoding sigma cross-reacting proteins of Escherichia coli
by: Smillie, David Andrew
Published: (1994)

Recombination at a 246 bp interrupted palindrome in Escherichia coli
by: Eykelenboom, John K.
Published: (2006)

Molecular characterisation of ESBLs from Klebsiella pneumoniae and Escherichia coli
by: Dashti, Ali
Published: (2002)

Cannot write session to /tmp/vufind_sessions/sess_mv198g6b1qk6430icnfv38898g