Cloning and expression of a human type V acid phosphatase single chain antibody fragment in Escherichia coli and Pichia pastoris
A recombinant single chain antibody (scAb) fragment specific for the isoenzyme human type V acid phosphatase has been expressed and secreted from Escherichia coli at a level of 0.02 mg/litre culture volume. Purification of the fragment using antibody immunoaffinity and metal chelate affinity chromat...
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University of Aberdeen
1996
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| Online Access: | http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320694 |