Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations

Droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling for simpler and faster PCR assay using wire-guided manipulations

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A computer numerical control (CNC) apparatus was used to perform droplet centrifugation, droplet DNA extraction, and rapid droplet thermocycling on a single superhydrophobic surface and a multi-chambered PCB heater. Droplets were manipulated using "wire-guided" method (a pipette tip was us...

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Bibliographic Details
Main Authors: You, David, Yoon, Jeong-Yeol
Other Authors: Department of Agricultural and Biosystems Engineering, The University of Arizona, Tucson, AZ 85721-0038, USA
Language:en
Published: BioMed Central 2012
Subjects:
Droplet manipulations
Escherichia coli
Peptidase D
Droplet PCR
Rapid PCR
Online Access:You and Yoon Journal of Biological Engineering 2012, 6:15 http://www.jbioleng.org/content/6/1/15
http://hdl.handle.net/10150/610171
http://arizona.openrepository.com/arizona/handle/10150/610171

Internet

You and Yoon Journal of Biological Engineering 2012, 6:15 http://www.jbioleng.org/content/6/1/15
http://hdl.handle.net/10150/610171
http://arizona.openrepository.com/arizona/handle/10150/610171

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