Novel tools for the study of protein-protein interactions in pluripotent cells

Unnatural amino acids (UAAs) have been used in bacteria and yeast to pinpoint protein binding sites, identify binding partners, PEGylate proteins site-specifically (vs. randomly), and attach small molecule fluorophores to proteins. The process of UAA incorporation involves the manipulation of the ge...

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Bibliographic Details
Main Author: Moncivais, Kathryn Lauren
Format: Others
Language:English
Published: 2013
Subjects:
Online Access:http://hdl.handle.net/2152/ETD-UT-2011-08-4097