Proteomic analysis of the pre-mRNA splicing machinery utilizing chromosomal locus epitope tagging in metazoans
Epitope tagging in metazoans is an important tool for biochemical analyses and is generally accomplished by using trans-genes with in-frame epitope tags. However, protein levels from trans-genes are rarely representative of native levels. To overcome the shortcomings using trans-genes, epitope tag...
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| Format: | Others |
| Language: | English |
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2008
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| Online Access: | http://hdl.handle.net/2152/3213 |