Application and Optimization of Bioluminescence Resonance Energy Transfer (BRET) for Real Time Detection of Protein-Protein Interactions in Transgenic <em>Arabidopsis</em> as well as Structure-Based Functional Studies on the Active Site of Coelenterazine-dependent Luciferase from <em>Renilla</em> and its Improvement by Protein Engineering
Bioluminescence resonance energy transfer (BRET) is a biological phenomenon in some marine organisms such as Renilla reniformis and Aequorea victoria. In BRET, resonance energy from decarboxylation of coelenterazine, a substrate of Renilla luciferase (RLUC), is transferred to its acceptor such as gr...
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Trace: Tennessee Research and Creative Exchange
2008
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| Online Access: | http://trace.tennessee.edu/utk_graddiss/355 |