Novel Methods for Synthesis of High Quality Oligonucleotides

Novel Methods for Synthesis of High Quality Oligonucleotides

The first part of the work describes a procedure of oligonucleotide purification using a reversed-phase cartridge. The developed method employs a very efficient yet mild oligonucleotide detritylation on the cartridge support allowing fast purification of oligonucleotides regardless of their 5´-modif...

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Bibliographic Details
Main Author: Semenyuk, Andrey
Format: Doctoral Thesis
Language:English
Published: Uppsala universitet, Institutionen för genetik och patologi 2006
Subjects:
Organic chemistry
oligonucleotide
solid-phase synthesis
RNA synthesis
phosphoramidite
abasic sites
depurination
2'-O-DTM
protecting group
cartridge
conjugate
dithiomethyl linker
base-stable linker
oligonucleotide purification
nucleoside
Organisk kemi
Online Access:http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7172
http://nbn-resolving.de/urn:isbn:91-554-6672-9

Internet

http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7172
http://nbn-resolving.de/urn:isbn:91-554-6672-9

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