Role of Multiple Glutathione Transferases in Bioactivation of Thiopurine Prodrugs : Studies of Human Soluble Glutathione Transferases from Alpha, Kappa, Mu, Omega, Pi, Theta, and Zeta Classes

Role of Multiple Glutathione Transferases in Bioactivation of Thiopurine Prodrugs : Studies of Human Soluble Glutathione Transferases from Alpha, Kappa, Mu, Omega, Pi, Theta, and Zeta Classes

A screening method was developed for identification of catalytically active enzymes in combinatorial cDNA libraries of mutated glutathione transferase (GST) derivatives expressed in E. coli. The method is based on spraying monochlorobimane (MCB) directly over bacterial colonies growing on agar. The...

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Bibliographic Details
Main Author: Eklund, Birgitta I.
Format: Doctoral Thesis
Language:English
Published: Uppsala universitet, Institutionen för naturvetenskaplig biokemi 2006
Subjects:
Biochemistry
Glutathione Transferases
Thiopurines
Prodrug
Bioactivation
Screening Method
Chemotherapy
Functional plasticity
Modulated activity
Biokemi
Online Access:http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7102
http://nbn-resolving.de/urn:isbn:91-554-6630-3

Internet

http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7102
http://nbn-resolving.de/urn:isbn:91-554-6630-3

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