Expression and Purification of Full-length CYP26 B1 and Spliced CYP26 B1

The goal of this project is to express both the normal CYP26 B1 and the spliced CYP26 B1 from human in Escherichia coli (E.coli) cells for further crystallization. This will be achieved by cloning in the DNA fragments into the Champion pET SUMO vector that is later transformed into E.coli cells. The...

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Bibliographic Details
Main Author: Sundin, Johanna
Format: Others
Language:English
Published: Örebro universitet, Akademin för naturvetenskap och teknik 2009
Subjects:
Online Access:http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-7394