The isolation and phenotypic characterization of Paramecium tetraurelia temperature-sensitive cell cycle mutants
Conditional cell cycle mutants which block cell division under restrictive conditions, increase their cell mass as a result of continued growth. Here, we develop a protocol to screen for large Paramecium temperature-sensitive cell cycle mutants by sizeselecting centrifugal elutriation. By using t...
Main Author: | |
---|---|
Format: | Others |
Language: | English |
Published: |
2009
|
Online Access: | http://hdl.handle.net/2429/3522 |