Topological analysis of the transhydrogenase in Escherichia coli membranes using proteolytic probes
Using proteolytic probes, the pyridine nucleotide transhydrogenase (EC 1.6.1.1) from Escherichia coli was analyzed for its native topography in the cytoplasmic membrane. Before analyses could be performed, the isolation of transhydrogenase-enriched ISO (inside-out) cytoplasmic membrane vesicles was...
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Language: | English |
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University of British Columbia
2010
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Online Access: | http://hdl.handle.net/2429/30401 |