Isolation of rat liver CTP: phosphocholine cytidylyltransferase and regulation of hepatic phosphatidylcholine biosynthesis

Two kinds of affinity chromatography, CDP-choline- and CTP-Sepharose 4B, were investigated for purification of the cytosolic CTP:phosphocholine cytidylyltransferase from rat liver. The enzyme did not show strong affinity for the CDP-choline Sepharose resin, but bound to the CTP-Sepharose column in t...

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Bibliographic Details
Main Author: Yao, Zemin
Language:English
Published: University of British Columbia 2010
Subjects:
Online Access:http://hdl.handle.net/2429/25073