Isolation and phosphorylation of guinea pig cardiac sarcolemma

Plasma membranes were prepared from guinea pig heart by homogenizing the tissue in a Polytron homogenizer, followed by four cycles of washing and differential centrifugation using low g forces (380 x g/10 minutes to 120 x g/10 minutes). The crude particles thus obtained were extracted with KCl (1.25...

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Bibliographic Details
Main Author: Hui, Chi Wai
Language:English
Published: 2010
Online Access:http://hdl.handle.net/2429/20102