Isolation and phosphorylation of guinea pig cardiac sarcolemma
Plasma membranes were prepared from guinea pig heart by homogenizing the tissue in a Polytron homogenizer, followed by four cycles of washing and differential centrifugation using low g forces (380 x g/10 minutes to 120 x g/10 minutes). The crude particles thus obtained were extracted with KCl (1.25...
Main Author: | |
---|---|
Language: | English |
Published: |
2010
|
Online Access: | http://hdl.handle.net/2429/20102 |