Collagenase and protease activity in Bacteroides melaninogenicus
A rapid sensitive collagenase assay has been developed using ¹⁴C-acetylated collagen as the substrate. Acid-soluble calfskin collagen was labelled with acetic-1-¹⁴C-anhydride at pH 8. The radioactively labelled collagen remained susceptible to Clostridium histolyticum collagenase but resistant to no...
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| Language: | English |
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2010
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| Online Access: | http://hdl.handle.net/2429/19379 |