Study on Antibacterial and Antioxidant Activity of Calendula Infused Oil

• Main Authors: WENG,YU-SHI, 翁鈺媐
• Other Authors: TSENG, YA-HSIU
• Format: Others
• Language: zh-TW
• Published: 2019
• Online Access: http://ndltd.ncl.edu.tw/handle/m8erhm

碩士 === 環球科技大學 === 生物技術研究所 === 107 === Calendula officinalis, a commonly known as marigold, is not only cultivated for ornamental purposes but is also used as edible flower for functional food due to its rich lutein and total tocophero1 contain, and high antioxidant properties. In this study, we compare the changes of the antioxidant properties of three types of table oils, germinated sesame oil, camellia oil, and sunflower oil before and after immersing air dried calendula petals. The amount of oil-soluble carotenoid was observed during immersing by a spectrophotometer method. Thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) was applied for the quantitative analysis of lutein, the results showed that each gram of calendula immersed in oil was able to dissolve 779.2 ± 40.0 μg of lutein. The quality index of oil antioxidant tests showed that the POV of the sesame oil with flower petals and the camellia oil with flower petals decreased significantly, while the POV of the sunflower oil with calendula petals showed no significant difference. According to the Ferric-reducing antioxidant power (FRAP) test, the antioxidant activity of the sunflower oil and camellia oil increased significantly after the addition of the flower petals, while there was no significance difference with germinated sesame oil. In the acid value (AV) and thiobarbituric acid (TBA) tests, there was no significant difference in the oils after the addition of the petals. In summary, from the analysis of the index of antioxidant capacity, although there was no consistent improvements, the oils with flower petals immersed has a positive effect on their color enhancement, nutrient increase, and oxidation resistance. In addition, the analysis the antibacterial activity of both aqueous extraction and ethanol extracts of the calendula petals, the result indicated that the aqueous extracts with no antibacterial effect on Escherichia coli ATCC25922, Staphylococcus aureus BCRC10451 and Candida albicans ATCC14053.