| Summary: | 碩士 === 靜宜大學 === 食品營養學系 === 107 === Based on the rise of people's health awareness, eating functional foods is a common phenomenon. Probiotic products are also widely accepted. In view of the good acceptance of chocolate and orange, this study is to investigate the LAB coated with orange juice and freeze-dried to probiotic orange powder, coated with chocolate to make probiotic chocolate, simulated gastrointestinal test to test the ability of LAB to resist acid and bile salts and the storage test to test the stability of products and LAB.LAB isolates PM212, GK12 and MP1 (The mixed strain of GK12 and PM212) was tested for Caco-2 cell adsorption test, Caco-2 cell and HepG2 cytotoxicity test, and simulated gastrointestinal test. Probiotic orange powder change the number of LAB during storage at 7 °C / 180 days less than 0.5 Log cfu/g, On the 30th day of the probiotic chocolate storage period, the number of LAB in the probiotic chocolate stored at 7 °C decreased < 1 Log cfu / g, while the LAB in probiotic chocolate stored at 25 ° C decreased about 7 Log cfu / g. The survival rate of MP1 was observed to higher than that of PM212 and GK12 at both storage temperatures of 7 ° C and 25 ° C after the end of the probiotic chocolate storage period. The results of acid and bile salt tolerance showed that the survival rate of LAB in PM212, GK12 and MP1 probiotic orange powser was better than that of pure bacteria the number of LAB maintaining at 105 CFU/g ~ 106 CFU/g. The probiotic chocolate coated PM212, GK12 and MP1 also showed good viability in the simulated gastrointestinal test, and the number of LAB was maintained at 107 ~ 108 CFU/g and the pure PM212, GK12 and MP1 number of LAB about 104 CFU/g after the simulated gastrointestinal test. The LAB adsorption capacity of Caco-2 cells was about 300 ~ 400 LAB/cell. High doses of PM212, GK12 and MP1 (108 Log cfu/g) had a cytotoxicity inhibition rate of 40-69% for Caco-2 and HepG2 for 12 hours, and a inhibition rate of 50-76% for 24 hours.The results showed that the LAB can effectively improve the tolerance after being coated with orange juice and chocolate. The number of lactic acid bacteria can be stabilized during storage at 7 °C. PM212, GK12 and MP1 have good Caco-2 cell adsorption, and the toxicity of cancer cells increases with dose and time.
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