| Summary: | 碩士 === 國立屏東科技大學 === 食品科學系所 === 104 === Many literatures reported that phenolic compounds is known to exhibit antioxidant and antithrombogenic effects and reduce the risk of cardiovascular diseases. Key point of the development of cardiovascular disease is according to vascular smooth muscle cell proliferation. The present study was to investigate the regulation of blood pressure by Pinus morrisonicola Hayata. Primarily, test samples were prepared by extraction in water, ethanol and ethyl acetate solvents or fermented by various sample/sugar ratio during 12 weeks periods.
Determination of antioxidant activity, including reducing power, DPPH radical scavenging activity and ABTS+ radical cation scavenging assays were conducted. Furthermore, total phenolic contents, flavonoid content and condensed tannins content were investigated. The results demonstrated that different treatment of DPPH radical scavenging capacity was between in 60-80%, ABTS+ radical cation scavenging rate was about 60% and reducing power reached up to 0.85 times of BHA equivalent. It showed good efficacy of inhibitory rate. The condensed tannins content suggest a time-dependent relationship. Total phenolic content of Pinus morrisonicola Hayata is 500-800 μg GAE / ml at 12th week. And flavonoids contents is 10-18 μg quercetin /ml at 12th week fermentation. The phenolic compounds and flavonoids in extracts are higher than those in ferments. Both of extraction and fermentation, illustrated that active ingredients analyzed by HPLC, abietic acid and β-sitosterol, are 91-243 ppm and 66-760 ppm observed respectively. Conclusively, our data showed the optimal fermentation period was three to four weeks. The concentration and antioxidant capacity of sample extract was positively correlated. Both extracts and ferments of Pinus morrisonicola Hayata could inhibit smooth muscle cell growth about 20-30%, and can effectively extend the activated partial thromboplastin time (APTT) more than 30 seconds, showing anticoagulant activity. Obviously, the development of health drink from Pinus morrisonicola Hayata is expected in the near future.
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