Dry-Reagent Strip Biosensor for Single Nucleotide Polymorphism in tmigd1 Gene

• Main Authors: Chih-WeiLin, 林志韋
• Other Authors: Jui-Chao Kuo
• Format: Others
• Language: zh-TW
• Published: 2016
• Online Access: http://ndltd.ncl.edu.tw/handle/26596697514819368124

碩士 === 國立成功大學 === 材料科學及工程學系 === 104 === In this research, we developed a lateral-flow immunoassay combined with primer extension (PEXT) method for SNP genotyping of tmigd1 gene in Tsaiya duck (Anas platyrhynchos), and discussed the effects of experiment parameters on both signal specificity and intensity. According to our result, the optimal setup is 1mM Mg2+ with 0.12μg streptavidin. Increasing the amount of PCR product or PEXT reaction cycle number can enhance the signal without observable change of signal specificity. To validate the reproducibility, we perform the test with additional 6 DNA samples. We were able to obtain specific signals from all the DNA samples, but the signal intensities were be affected by the concentration of PCR prducts. According to our calculation, time requirement of this method is about 40 minutes, and the cost for 1 strip test is about 38.5 NTD.