| Summary: | 碩士 === 國立虎尾科技大學 === 生物科技研究所 === 102 === Many of functional food are developed from natural sources, and Chinese herbal medicine is an important one. The products, containing the Rhodiola, have also been developed in the market.
Rhodiola, belong to Crassulaceae family, is a perennial herb and distribute in Bhutan, Sikkim, Nepal and China''s Tibet, Sichuan and Yunnan. The Rhodiola genus has more than 200 plants around the world. The aims of this thesis were studying on the constituents and bioactivities of Rhodiola genus, systematically, and preparation methods and pharmacological activities of main active components, salidroside. It includes Rhodiola category and distribution, phytochemicals, and pharmacological activities. The phyochemical study have reported that this genus contained flavonoid, volatile oils, glycosides, coumarins, steroid, tannins, organic acids, phenols, terpenoids, fats and proteins. The crude extract of Rhodiola exhibited anti-fatigue, anti-anoxia, anti-aging, anti-virus, anti-tumor, anti-microwave radiation and detoxcification. The bioactivities of salidroside exhibited ant-oxidative, anti-anemia, anti-diabetes, reduce oxidative stress, detoxification, and neuron protection effects.
The alcohol precipitation was optimal method than lead acetate precipitation for preparation of salidroside, and using ultrasonic technology can also improve the alcohol precipitation method. The optimal temperature was 60 ℃for ultrasonic extraction under thermostatic conditions. Finally, the crude extract was subjected on column chromatography to obtain salidroside.
In this thesis, we also established the rapid preparation method for salidroside purification in large scale. The powder of R. crenulata was extracted with 95% ethanol, then, the extract was partitionated with different polarity solvents. The sublayer, containing salidroside, was subjected on column chromatography to yield salidroside. Quantitative this method maybe provide the purification method for salidroside for large-scale in the future.
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