| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 102 === The aim of this study is to hydrolyze hot acid extracted okara polysaccharide (PS) by crude enzymes produced by tempeh mold isolates, analyze the composition of oligosaccharides (OS) products and preliminary biological activities. Five strains of tempeh mold IDPT-YH-1, IDPT-YH-2, IDOT-YH-3, IDST-YH-4, and TWNT-YH-5 were isolated from Indonesian peanut tempeh, soya bean okara tempeh, soy tempeh, and tempeh produced in Taiwan. The mannase activity were 2.42, 5.48, 2.45 U and the endocellulase activity were 1.52, 2.61, 1.85 U from strain IDPT-YH-2, IDST-YH-4, and Rhizopus oligosporus BCRC 31996 with 50oC and pH 5 as optimal reaction conditions. HPLC analysis of < 3 kDa okara acid hydrolysate showed that there are increase in time and small molecule OS peak area, after crude enzyme hydrolysis. The protein, formaldehyde nitrogen, ammonia-type nitrogen and amino-type nitrogen content of IDPT-OS-5, IDST-OS-2, and RO-OS-2 were 0.13, 0.27-0.54, 0.11, and 0.16-0.48 mg/mL. TLC analysis of okara hot acid hydrolyzate have the same sugar spot with raffinose and stachyose standards and after hydrolysis by IDPT-YH-2, IDST-YH-4, or R. oligosporus BCRC 31996 crude enzymes, the previously detected sugar dark spot become lighter. Preliminary estimated that raffinose would become small oligosaccharides and monosaccharide after crude enzymes hydrolysis produced from mold strain. Compared to IDPT-OS-5 and RO-OS-2, IDST-OS-2 has better anti-oxidation activity, that DPPH radical scavenging capacity was 92.86 ± 0.06%, equivalent to 49.36 ± 0.10 g/mL Trolox; reducing power OD700nm was 0.65 ± 0.01, equivalent to 32.35 ± 0.50 g/mL Trolox; ferrous ion chelating ability was 63.35 ± 0.85%. IDPT-OS-5 can increase Bifid. adolescentis, B. bifidum, Lb. plantarum, and Lb. acidophilus bacterial count to 7.85, 7.97, 8.15, and 8.34 log cfu/mL, higher than control with no OS (6.37-7.74 log cfu/mL). IDST-OS-2 also can increase Bacillus licheniformis FRI MY-55 bacterial count to 7.61 log cfu/mL, higher than control with no OS (7.37 log cfu/mL). The angiotensin I converting enzyme (ACE) inhibitory activity of IDPT-OS-5, IDST-OS-2, and RO-OS-2 were 5.44 ± 0.48%, 4.30 ± 0.86%, and 6.02 ± 0.67%, respectively.
|
|---|
