| Summary: | 碩士 === 嘉南藥理大學 === 保健營養系 === 102 === The worldwide prevalence of hypertension continues to increase, the primary prevention of hypertension has become an important global public health initiative. Dietary intake of phenolic compounds is inversely proportional to the risk of chronic diseases (including cardiovascular disease and certain cancers). However, better results in the classification of phenolic compounds are not yet clear. The effects of selected phenolic compounds on the mechanism of antihypertensive activity in mouse vascular endothelia cell line (SVEC) and angiotensin converting enzyme (ACE) activity were investigated. The phenolic compounds include three flavones such as apigenin, baicalein and chrysin, seven flavonols such as galagin, myricetin, fisetin, kaempferol, quercetin, rutin and morin, and four phenolic acids such as gallic acid, chlorogenic acid, caffeic acid and ferulic acid. The results indicate that all the phenolic compounds except gallic acid and caffeic acid show no cytotoxicity to SVECs. Out of these phenolic compounds, myricetin, quercetin, rutin and chlorogenic acid demonstrate significant induction on the levels of NO and PGI2 as well as on eNOS and COX-2 activities. These four phenolic compounds show marked inhibitory effect on ROS generation in H2O2-induced SVECs. In addition, these four phenolic compounds significantly inhibit ACE. Enzyme kinetic analysis reveals that myricetin and chlorogenic acid is all a noncompetitive inhibition of ACE. The presence of hydroxyl groups on C-5 in the A-ring, and on C-3', C-4' in the B-ring of flavonoids, or more hydroxyl group in phenolic acids enhanced antihypertensive activity. In conclusion, myricetin, quercetin, rutin and chlorogenic acid showed better antihypertensive activity in SVEC and ACE inhibition.
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