| Summary: | 博士 === 國立臺灣大學 === 微生物與生化學研究所 === 98 === Strain NTU 568 was a Monascus isolated from red mold rice by our laboratory. In our previous study, strain NTU 568 has several functions of reducing blood serum lipid, antifatigue, decreasing amyloid peptide accumulation, prevention of obesity and inhibiting lung cancer cell migration. The morphological observations, which include colonies size, ascospores size, and the partial sequences of ribosomal RNA genes- the internal transcribed spacer 1 (ITS1), 5.8S ribosomal RNA gene, internal transcribed spacer 2 (ITS2), and 18S ribosomal RNA gene, were applied to identify the strain NTU 568. Comparisons of partial nucleotide sequences and morphology with the strains from different genus (M. purpureus, M. ruber and M. pilosus) were made. The DNA sequences data demonstrated that strain NTU 568 was similar to BCRC 31615 (M. purpureus). The morphological observations and DNA sequences data indicated that strain NTU 568 and BCRC 31615 were the same species but not same strain. Further study using strain NTU 568 was employed.
The approaches of this research are investigating on arise the secondary metabolites by different substrates and the effects of Monascus-fermented products on improvement diabetes symptoms in animal model. In the current study, we examined the effect of Monascus fermented products (red mold rice, red mold adlay and red mold dioscorea) on oral glucose tolerance testing in streptozotocin-induced diabetic Wistar rats. After 8-weeks feeding with Monascus fermented products at a dose of 200 mg/kg, the experimental results indicated that Monascus fermented products could delay the development of plasma glucose level in diabetic rats. Diabetes shares a common pathophysiology that may be explained as a consequence of hyperglycemia-mediated over-production of reactive oxygen species. The diabetic control rats showed higher reactive oxygen species and lower activities of glutathione disulfide-reductase, glutathione reductase and catalase in pancreas as compared to Monascus fermented products-treated rats. The results indicated that Monascus fermented products not only regulate hyperglycemia but also provide the preventive Hypothetical diagram on the antidiabetic/antioxidative/anti-inflammatory effects of red mold fermented products. effects of STZ-induced pancreatic damage. Red mold dioscorea has better effect than red mold rice and red mold adlay.
Diabetic complications have demonstrated that the production of excess reactive oxygen species and inflammatory factors. Both oxidative stress and inflammation play a major role in the development of tissue insulin resistance. Red mold dioscorea contains various antioxidants considered useful for the treatment of diabetes. Throughout the experimental period, significantly lowered plasma glucose, triglyceride, cholesterol, free fatty acid and low density lipoprotein levels were observed in the red mold dioscorea-treated groups. Red mold dioscorea also inhibited diabetes-induced elevation in the levels of interleukin (IL)-1β, IL-6, interferon-γ and tumor necrosis factor-α. These results indicate that red mold dioscorea could attenuate diabetes and thus, minimize diabetic complications via the combination of its anti-hyperglycemia, anti-oxidative and anti-inflammatory effects. Monascus will be developed as a multi-functional food in the future.
|
|---|
