Studies on the antibacterial activity, antioxidation,anti-inflammation and anticancer activity of Wedeliatrilobata (L.) Hitchc.

• Main Authors: Lai Chung Wei, 賴忠維
• Other Authors: 韓建國
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/07851498602097719470

碩士 === 亞洲大學 === 保健營養生技學系碩士班 === 97 === Abstract Wedelia trilobata (L.) Hitchc. is used in folk medicine. It is reported that the plant shows antibacterial activity and contents antitumor chemical components. The purpose of this research was to evaluate antibactes, antioxidation, anti-inflammation, and anticancer activity of W. trilobata. W. trilobata flowers , leaves and stems were extracted with ten times of ethyl alcohol. The extract was then partitioned by n-Hexane, ethyl acetate, n-butyl alcohol and water to evaluate its antimicrobial and antioxidative activities. We also evaluate its anti-inflammation and anticancer activity of the flower. The result showed that most extracts had antimicrobial activities except the water extracts from flower. The ethyl acetate extract was the most effective among all the extracts. Most extracts from flowers, leaves and stems showed the good antioxidative activities especially the flower. The the ethyl acetate extracts presented the best on DPPH scavenging activity and reducing power and had the highest total phenolic compounds content . We used the ethyl alcohol and water extracts of W. trilobata to treat RAW264.7 macrophage, which induced inflammation by LPS. In the NO2 assay, the extracts of W. trilobata flower had better inhibitory ability .In transient transfection assay shows the n-hexane and ethyl alcohol extracts of W. trilobata flower can activate PPARα. In MTT assay of SK-Hep-1, we found that extract of W. trilobata flower had the best inhibitory ability. We also found the ethyl alcohol extract of W. trilobata had the best ability to diminish the expression of matrix metalloproteinase (MMP)-9 and MMP-2. The ethyl alcohol extracts of flower had good anti-migration and anti-invasion ability especially on 80 μg/mL dose.