Effect of polyphenol-rich milk powder on anti-aging in senescence accelerated mice

• Main Authors: Chia-jung Wu, 吳佳蓉
• Other Authors: Ming-fu Wang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/33719464184340479039

碩士 === 靜宜大學 === 食品營養研究所 === 97 === Previous studies indicated that EGCG is the major and most active component of green tea catechins, and it acts as an antioxidant in the biological system. The purpose of our study was to investigate the effects of polyphenol-rich milk powder composed mainly of EGCG on learning and memory ability, and antioxidant status in 3 and 6-month-old senescence accelerated mice (SAMP8). SAMP8 mice were devised to four groups: control, 1.04 mg, 2.08 mg and 5.20 mg polyphenol (PP). Each group was consisted of twenty male and female mice. During feeding period, intake amount, body weight measurement and aging scores were recorded. Locomotion was recorded in evaluation of behavior; and single-trail passive avoidance test and active shuttle avoidance test in evaluation of learning and memory abilities. After the experiment, the mice were sacrificed to analyze the biochemical parameters, the antioxidant ability of serum, liver and brain, and the histopathological examination of brain were also observed and evaluated. Our results showed that there was no significant difference in the body weight, food intake, and locomotion among four groups in 3 and 6-month-old mice. The aging score were increased with age, and the score of control groups were higher than the experimental groups in both male and female 3 and 6-month-old mice (p<0.05). The experimental groups had better active shuttle avoidance response in both male and female 3 and 6-month-old mice (p<0.05). 3 and 6-month-old male and female mice fed with polyphenol had significantly better single-trial passive avoidance results (p<0.05). 3 and 6-month-old male and female mice fed with polyphenol experiment diet had significantly lower triglycerol, total cholesterol than control group (p<0.05). Hepatic protein carbonyl levels, and malondialdehyde concentration and Aβ deposition of brain were significant lower in the experimental groups than in control ones in 3 and 6-month-old male and female mice. Polyphenol also brought about an augmentation in the activities of enzymic antioxidants in the liver, such as superoxide dismutase, catalase, glutathione peroxidase and total thiol levels, and increased the activity of glucose-6-phosphate dehydrogenase in the blood. In summary, we conclude that the consumption of polyphenol-rich milk powder composed mainly of EGCG may improve the learning and memory ability of SAMP8 mice. And it may due to the mediation by EGCG promoting the antioxidative ability and reducing the brain pathological changed that lower the oxidative injury.