Study of the effect of nano-gold and buffer on natural-convection polymerase chain reaction

• Main Authors: Wei-tsang Chiou, 邱偉倉
• Other Authors: Ming-Jyh Chern
• Format: Others
• Language: zh-TW
• Published: 2009
• Online Access: http://ndltd.ncl.edu.tw/handle/09618131102317338822

碩士 === 國立臺灣科技大學 === 機械工程系 === 97 === We investigate the effects of PCR (Polymerase Chain Reaction) buffer, such as Deionized water (DI water), TBE buffer and nano gold colloid, on PCR. A conventional PCR and natural-convection PCR are considered to explore the effects. Electrophoresis was conducted to analyze the PCR product. We found that the amount of PCR product without any buffer and specificity were changed when the number of cycle of PCR increased. In addition, we found that adding the TBE buffer can keep the specificity even the number of PCR cycle exceeds 35 cycles. On the other hand, nano gold colloid and its buffer restrained PCR, but they still kept the specificity. It is, however, revealed that the enough PCR yield was obtained by diluted nano gold colloid. Subsequently, we utilized a PIV (Particle image velocimetry) technique to observe the flow structure of natural-convection PCR. The mechanism why natural-convection PCR is more efficient than conventional PCR was studied. The variation of flow structure of natural-convection PCR due to nano gold colloids was found in PIV results.