| Summary: | 碩士 === 國立臺灣科技大學 === 機械工程系 === 97 === We investigate the effects of PCR (Polymerase Chain Reaction) buffer, such
as Deionized water (DI water), TBE buffer and nano gold colloid, on PCR. A conventional PCR and natural-convection PCR are considered to explore the effects.
Electrophoresis was conducted to analyze the PCR product. We found that the
amount of PCR product without any buffer and specificity were changed when the
number of cycle of PCR increased. In addition, we found that adding the TBE
buffer can keep the specificity even the number of PCR cycle exceeds 35 cycles. On the other hand, nano gold colloid and its buffer restrained PCR, but they still kept the specificity. It is, however, revealed that the enough PCR yield was obtained by diluted nano gold colloid. Subsequently, we utilized a PIV (Particle image velocimetry) technique to observe the flow structure of natural-convection PCR.
The mechanism why natural-convection PCR is more efficient than conventional
PCR was studied. The variation of flow structure of natural-convection PCR due
to nano gold colloids was found in PIV results.
|
|---|
