The effect of modified jelly fig pectin and its hydrolysates on human stomach cancer cells (AGS)

• Main Authors: Szu-Yu Pan, 潘思妤
• Other Authors: Ean-Tun Liaw
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/92438099269515951077

碩士 === 國立屏東科技大學 === 食品科學系所 === 97 === A nutrient of recent interest to cancer researchers is citrus pectin. However, citrus pectin is modified (MCP) by adjusting the pH that breaks it into shorter, non-branched, galactose-rich chain that are better absorbed and utilized by the body. Previous studies have shown that the modified citrus pectin could definitely inhibit lots of tumor cells. Furthermore, the studies have indicated that pectin also can induce cancer cells undergoing apoptosis. Therefore, the aim of present study is to investigate the cancer cell inhibition by modified jelly fig pectin and its hydrolysates for promoting its added-values. By MTT assay, six hydrolyzed samples were prepared and they showed effective inhibition on AGS stomach cancer cells. The inhibition rate can be achieved up to 50% when the tumor cell treated with these samples for 72 hours. By Reverse Phase microscope, cell apoptosis morphology was observed. Simultaneously, cell cycle analysis, early apoptosis, and caspase-3 activation were conducted by flow cytometry. In cell cycle, the results indicated that the sub-G1% could reach 22-36% after treated with all of the samples for 72 hours. In early apoptosis investigation, the data suggested that 11-30% early apoptosis can be found when hydrolyzed samples were employed for 48 hours. Moreover, in caspase-3 activity assay demonstrated that the caspase-3 was activated after treating with specified samples. Conclusively, jelly fig pectin modified with alkaline and enzymatic treatment exhibited apoptosis pathway on stomach cancer cells.