On-column refolding of a recombinant protein with immobilized metal affinity adsorbents

碩士 === 國立中興大學 === 化學工程學系所 === 97 === Over-expression of recombinant GlcNAc 2-epimerse in Escherichia coli system was formed with inclusion body by 80%. In this study, the Ni-NTA immoblilized metal affinity column was applied to high-pH and urea solubilization systems for investigating the effect of...

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Bibliographic Details
Main Authors: Beng-chung Li, 李秉忠
Other Authors: Sung-Chyr Lin
Format: Others
Language:zh-TW
Published: 2009
Online Access:http://ndltd.ncl.edu.tw/handle/66898753031335483543