| Summary: | 碩士 === 朝陽科技大學 === 生物技術研究所 === 97 === Verticillium lecanii was generally recognized as a pathogenic fungus that is widely spread in nature and is parasitic in many insects of agricultural concerns. The fungus has drawn many interests of developing into microbial insecticide in the biological agricultural chemical industry. The intentions of this research are: optimizing the fermentation conditions for spore production, finding a practical method for controlling the sporulation in order to maintain the viability, and investigating the mass and heat balance of the solid state fermentation. For cultures in plastic pouches, induction of sporulation at 30°C for 2 days,at the end of fermentation, spore production of 1.76×1010 spore/g was obtained. The germination rate was 61.46%. For cultures in a 10 liter fermentor, induction of sporulation at 28
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