The Functional Studies of Antioxidant Ability and Antihypertension with Soft-shell Turtle Powder

• Main Authors: Shu-Yun Wang, 王姝勻
• Other Authors: Li-Yun Liu
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/69483149613554606262

碩士 === 實踐大學 === 食品營養與保健生技研究所 === 96 === Soft-shell turtle (SST) is one kind of traditional food that good for health. It contained 62.3 % crude protein, 2.0% crude fat, 5.05% water and 30.8 % ash. SST powder was extracted by different solution to obtain different characteristics protein. We determined the free radical scavenging ability, reducing power and iron chelating ability of the extracted solutions. The DPPH scavenging ability of the 0.5M NaCl extacted solution was the best. Water extraction did not increase in different extraction time. The condition of the optimal incubation temperatures for bromelain, ficin and papain were 60℃, 60℃and 65℃, respectively. Best hydrolysis acid-base environment of bromelain, ficin and papain individually at pH 7, pH 9 and pH 8. The suitable ratio of the enzyme and substract was [ E / S = 1 / 2000 ] . The ACE IC50 of the papain and ficin hydrolysate was 2.30 mg/mL and 1.99 mg/mL , individually. After dialysis, the molecular weight more than 3500Da of papain and ficin hydrolysate ACE inhibition IC50 was 3.02 mg/mL and 4.36 mg/mL, molecular weight less than 3500Da, ACE inhibition IC50 was 4.51 mg/mL and 5.21 mg/mL, individually. SHR was treated with different dosage of papain hydrolysate molecular weight less than 3500Da by oral administration. After 8 weeks, blood pressure of high dosage group was showed significantly lower than control group, the change of pressure close to positive control group.