Behavior of Chitosan Oligosacharides Addition on Amperometric Glucose Biosensor Performance

• Main Authors: Benny Gosali, 吳班尼
• Other Authors: Chiapyng Lee
• Format: Others
• Language: en_US
• Online Access: http://ndltd.ncl.edu.tw/handle/09596170132843407938

碩士 === 國立臺灣科技大學 === 化學工程系 === 96 === ABSTRACT An amperometric glucose biosensor based on screen printed carbon electrode (SPCE) was successfully developed in this research. Carbon electrode and insulation layer was screen-printed onto PVC substrate. Reagent layer which contained ferricyanide and chitosan oligosaccharides was also screen-printed on the defined area. The glucose oxidase solution was applied to the reagent layer by drop-coating technique. Adhesive layer was stencil-printed onto the SPCE to form the spacer and define the sample chamber. Finally application of the hydrophilic layer to the glucose biosensor completed the whole fabrication process. The physical characteristics of the SPCE and glucose biosensor were studied by optical microscope (OM), and scanning electron microscope (SEM) technique, while their electrochemical characteristics were studied by cyclic voltammetry and amperometry technique. Interference and stability tests were also done in this research. The fabricated glucose biosensor exhibited a relatively fast response (below 10s), and only 1.5μL sample needed. Its sensitivity was 0.027μA/mg/dL, and had linear detection range from 50-600mg/dL. The average coefficient of variation was 5.7%. No interference effects from oxygen and ascorbic acid (1.5mg/dL) were observed during the interference tests. After 55 days, glucose biosensor retained 93% of its initial activity. Chitosan oligosaccharides addition into the glucose biosensor’s reagent layer successfully increased its sensitivity and stability.