Essential Oil Extraction of Citrus microcarpa Bonge and Its Bioactivity

• Main Authors: Tsyr-Ling Chang, 張慈玲
• Other Authors: Tzou-Chi Huang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/66478789770024425079

碩士 === 國立屏東科技大學 === 食品科學系所 === 96 === This investigation used the fully ripened Citrus microcarpa Bonge as the raw material for essential oil extraction. Extracts of essential oil from the peels were conducted expression, steam evaporating, and reduced pressure steam distillation, respectively. Yield and the quality of the extracts were studied to evaluate these three methods. Bioactivities including DPPH scavenging capability and bacteriostasis were conducted on the extracted essential oils. Bacteriostatic ability were performed on Escherichia coli, Pseudomonas aeruginosa, and Enterococcus faecalis. Finally, GC-MS, and NMR were used to confirmed the primary composition of essential oils from Citrus microcarpa Bonge. The results verified that reduced pressure steam distillation is the best extraction method among these three methods applied, judging by the yield and the quality of essential oils. The best DPPH scavenging ability was found with 2.0% of essential oil (31.0%). The data from bacteriostasis showed that 2.0% of essential oil had the best inhibition on Enterococcus faecalis (diameter of bacteriostatic ring was 15.8 mm). What came next was that 1.5% of essential oil on Pseudomonas aeruginosa (ring diameter was 13.7 mm). The inhibition of 2.0% essential oil on Escherichia coli was found to be moderate (ring diameter was 12.8 mm). Finally the primary composition of Citrus microcarpa Bonge essential oil was confirmed as limonene.