Effects of cis- and trans-Cinnamic Acid on the Lipid Profiles and Antioxidant Protein Levels in Rats Fed with High Cholesterol Diets

• Main Authors: Chih-Jung Ho, 賀智瑢
• Other Authors: Fang-Ming Sun
• Format: Others
• Language: en_US
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/46877589209451583603

碩士 === 嘉南藥理科技大學 === 營養與保健科技研究所 === 96 === The purpose of this study was to investigate the effects of cis- and trans- cinnamic acid (c-CA and t-CA) supplementation on the lipid metabolisms and antioxidant systems of rats fed a high-cholesterol diet in vivo. The c-CA was prepared and purified by the method developed by Sun et al. (2003). The rats (n = 28) were randomly assigned to four groups and given a diet containing 1.2 g cholesterol/kg for 5 weeks. The control group only received a high cholesterol diet, whereas the other three groups received a diet including t-CA or Vitamin C (Vit C)or c-CA (0.1 g/100 g). The total cholesterol concentrations (Total-C), high-density lipoprotein-cholesterol concentrations (HDL-C), triglyceride concentrations (TG), atherogenic index (AI) and thiobarbituric acid reactive substances level (TBARS) of plasma and/or hepatic samples were determined to test the effects of c-CA on the lipid metabolisms of rats. The levels of hepatic antioxidant enzymes (catalase, and superoxide dismutase) were also evaluated by Western blot analysis. The plasma Total-C was significantly lowered in the Vit C and c-CA than in the control group. The plasma ratio of HDL-C/Total-C was significantly higher in the Vit C than in the t-CA group, so the AI was reversed in these two groups. The plasma HDL-C and plasma TG were not appreciably different among four groups. According to this study, the hepatic TBARS significantly was reduced in the c-CA than in the Vit C group. The hepatic TBARS was significantly lowered in the c-CA than in the Vit C group. The hepatic Total-C was significantly lowered in the control than in the t-CA group. The hepatic TG was significantly lowered in the control than in c-CA. There were no significant differences in the levels of hepatic antioxidant enzymes (catalase and superoxide dismutase) among the four groups. These results indicate that c-CA was more effective in lowering the plasma cholesterol and hepatic TBARS content than t-CA or Vit C.