| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 95 === Fu-Lin (Poria cocos) is one of the most important traditional medicines in China. The aims of this research are to enzymatic degradation of Fu-Lin, followed by fermentation of the fu-Lin hydrolysate with lactic acid bacteria (LAB), and to evaluate the immunomodulatory and ontioxidative activity of the LAB-fermented products. Cellulase was chosen to degrade the polysaccharides of Poria cocos for the production of a water-soluble hydrolysate with immunomodulation activity. The optimal concentration and digestion time for cellulase action on 3 % Fu-Lin were 50 U/mL and 15 hours, respectively. The obtained Fu-Lin hydrolysate significantly increased the superoxide anion production and enhanced phagocytosis activity from 24% to 53% in RAW 264.7 macrophages. Among 11 LAB strains, Lb. acidophilus BCRC 10695, Lc. lactis subsp. Lactis BCRC 12315, Lb. rhamnosus BCRC 14068,Lb. rhamnosus GG (LGG), Lb. plantarum BCRC 10069 and BCRC 12250 grew in 2% Fu-Lin hydrolysate and their cell count could reach 7.5 Log CFU/mL after 6 hours. The cell count of this LAB strains in 1 % Fu-Lin hydrolysate with addition of 1% Soytone, Casamino acid and Skim milk could increase to 8Log CFU/mL after 9 hour incubation. The products fermented by strains of BCRC 10695, BCRC 12315or LGG strains in 1% Fu-Lin hydrolysate with 1% Soytone singnificantly enhanced the cell proliferation of RAW 264.7 macrophages. The cell growth was faster in two combining strains, compared to that inindividual strain only. The producted fermented by 2 combining strains in 1 % Fu-Lin hydrolysate containing 1 % soytone significantly increased the cell proliferation and phagocytic activity of RAW 264.7 macrophages. The products also significantly increase the proliferation of peripheral blood mononuclear cells cells and the activity of natural kiler cells. The drink fermented by combining strains of LGG and BCRC 12315 was show to have the highest antioxidative activity, especially, on ferric ion reducing and DPPH scavenging activity. However, this product had limited activites on chelating Fe2+ ion and inhibition the autoxidation of ascorbic acid.
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