Effect of microencapsulated on activity of fibrinolytic enzyme

• Main Authors: Yea-Ling Yang, 楊雅玲
• Other Authors: Pao-Chuan Hsieh.Ph.D.
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/cyb4bt

碩士 === 國立屏東科技大學 === 食品科學系所 === 95 === Bacillus subtilis var. natto was isolated from Japan and Taiwan commercial natto products. Bacillus subtilis var. natto could produce high fibrinolytic enzyme activity (nattokinase) that degraded fibrin. The cultivation of Bacillus subtilis var. natto was investigated for the increasing fibrinolytic enzyme production. Shaking conditions, inoculum sizes, carbon sources, nitrogen sources, mineral salts, initial pH values and culture temperatures were optimized for fibrinolytic enzyme production from Bacillus subtilis var. natto. The results showed that 50 ml medium in 250 ml Hinton’s, which contained 2.5% Potato Starch, 3% Beef Extract, 0.25% K2HPO4 and the medium initial pH 7, shaked speed with 150 rpm and 2.5%(v/v) inoculums size producing the highest fibrinolytic enzyme activity (129 FU/ml) after cultured at 37℃ for 36 hours. An improved method of microencapsulation was developed to increase the efficacy of capsules in protecting the encapsulated enzyme under simulated gastric conditions. The effects of different capsule sizes, different sodium alginate concentrations, different concentrations of calcium chloride and the hardening time on the viability of encapsulated enzyme were investigated. Expect that the encapsulation method described in this study may be effectively used to protect the fibrinolytic enzyme from adverse gastric conditions. The results showed that the optimum conditions are needle size 1.2 mm, mixing ratio 1:4 of fibrinolytic enzyme and 1.5% alignate, 0.15 M CaCl2 and hardening time 1 hours. The microencapsulation method can protect the fibrinolytic enzyme activity from adverse gastric conditions and keep the activity. Microencapsulated fibrinolytic enzyme can more keep the activity above 0℃after storing one month than unmicroencapsulated.