Effect of formulations and drying conditions on the viability of powdered lactic acid bacteria.

• Main Authors: Wei-min Kao, 高唯敏
• Other Authors: Chee-Shan Chen
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/94811516845957577135

碩士 === 朝陽科技大學 === 生物技術研究所 === 95 === This research studied the submerged fermentation, medium composition, formulations and drying process for the production of lactic acid bacteria. The application of a trial, low temperature shelf-type drying system on the powdered product was also studied. The aim of this work was to improve soymilk cultural medium compositions and using four kinds of formulations for drying process. Three strains: Lactobacillus acidophilus, Bifidobacterium bifidum and Lactobacillus sporogenes were used in this study. Using improved fermented soymilk for flask culture, the cell concentration of Lactobacillus acidophilus was 5.27 ×1010 CFU/mL（30 ℃, 12.5% soymilk powder, 800 ppm bromelain, ends at 24hr）; while that for Bifidobacterium bifidum was 4.32×109 CFU/mL（28 ℃, 10 % soymilk powder, 1000 ppm bromelain, ends at 24 hr）. For Lactobacillus sporogenes, the cell concentration was 2.28×1010 CFU/mL （33 ℃, 12.5 % soymilk powder, ends at 24 hr）. In drying process, the trial, low temperature shelf-type dryer could produce lactic acid bacteria powder, at drying temperature of 35°C, in 3 HR. The cell viability of Lactobacillus acidophilus powder were 4.82×107, Bifidobacterium bifidum powder were 7.38×108 , and that for Lactobacillus sporogenes powder was 5.67×107 . The survival of lactic acid bacteria powder after drying process, Lactobacillus acidophilus powder were 83.15%, Bifidobacterium bifidum powder were 81.65%, and that for Lactobacillus sporogenes powder was 84.99%. The present study has showen that low temperature shelf-type drying system could reduce the loss of cell viability during drying.