Summary: | 碩士 === 靜宜大學 === 食品營養研究所 === 94 === Catechins were demonstrated to have antioxidant and redusing blood glucose capacity. The purpose of this study was to investigate the effect of catechins on learning and memory ability, antioxidative status, blood glucose and insulin content in senescence accelerated mice. 3- and 6-month-old senescence accelerated male and female mice were divided into four groups: control group, 50, 200 and 800 ppm aqueous solutions of catechins(experimental groups). After 12 weeks of feeding, body weight, food intake, drink amount, aging score, open field activity test, single-trial passive avoidance and active shuttle avoidance test were performed during the experiment. The oral glucose tolerance test (OGTT) was analyzed before sacrificed. The biochemical parameters of serum were analyzed after sacrificed. The activity of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione reductase, glucose-6-phosphate dehydrogenase (G-6-PD), malondialdehyde (MDA) , protein carbonyl and total thiol concentrations and insulin content, pathological examination of brain and pancreas. The results showed that there were no significant differences in the body weight, food intake, drink amount and locomotion among four groups. The aging score of experimental groups were significantly lower than the control group in the 3-and 6-month-old mice (p<0.05). In learning and memory test, experimental groups of 3-and 6-month-old senescence accelerated male and female mice had significantly better in active shuttle avoidance response and single-trial passive avoidance test (p<0.05). The results of OGTT, experimental groups of 3-and 6-month-old senescence accelerated male and female mice had significantly lower than the control group on blood glucose at 0, 30, 60, 90, 120 minutes (p<0.05). In concentration of plasma insulin, experimental groups had significantly lower than the control group in before and 30, 60, 90, 120 minutes (p<0.05). The SOD activity was no significantly different in both 3-and 6-month old male and female mice. The catalase, GPx, glutathione reductase, G-6-PD activity and total thiol concentration, experimental groups of 3-and 6-month-old senescence accelerated male and female mice had significantly higher than the control group (p<0.05). The MDA and protein carbonyl concentrations in the experimental groups were significantly lower than the control group. The β-amyloid protein deposition of brain, pancreas and insulin content were no significantly different in the 3-and 6-month male and female mice. From the findings of these results, the supplement of catechins may ameliorate learning and memory ability, antioxidative system and increase insulin sensitivity in SAMP8 mice.
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