Elucidating Physiologic Effects of Milk Fish at Low Temperature by Proteomic
碩士 === 國立高雄海洋科技大學 === 水產食品科學研究所 === 94 === The milk fish usually is cultured in Subtropical area at middle Taiwan for its vulnerable effects in temperature below 15°C. The massive death often occurred during winter to bring large economic loss. The physiologic stress of fish creatures will occur whe...
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| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2006
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| Online Access: | http://ndltd.ncl.edu.tw/handle/93158413381431266408 |
| Summary: | 碩士 === 國立高雄海洋科技大學 === 水產食品科學研究所 === 94 === The milk fish usually is cultured in Subtropical area at middle Taiwan
for its vulnerable effects in temperature below 15°C. The massive death
often occurred during winter to bring large economic loss. The physiologic
stress of fish creatures will occur when the dramatically changes of
temperature such as extreme hot or cold. There are many hormone or
regulatory proteins secrete in cells when physiologic stress occur. The
present study intend to clarify the change of plasma glucose and lactate as
well as the changes of serum and muscle proteins in milk fish during cool
temperature.
To separate each protein more identifiable in 2D electrophoresis、the
muscle was pretreated by 2D sample buffer and the serum was pretreated by
rehydration buffer. The concentrations of plasma glucose and lactate
increased significantly to peak at 48 hours and then decline to the normal
level. The obviously changes of proteins occurred after treating with cool
temperature even more some specific proteins induced. Whether some
unknown proteins are induced will be clarified by the comparisons of the
amount and types of proteins in serum and muscle during normal
temperature and cool temperature. Each newly induced protein which
graphically marked by staining and recognized by Image Master can be
identified by MALDI-TOF.
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