| Summary: | 碩士 === 國立中正大學 === 化學工程所 === 94 === Abstract
Lactate is one of the major fermented products from lactic acid bacteria. Furthermore, diacetyl is a small amount of valuable byproduct for dairy food and pharmaceutical application. In the past decade, the reserchers only focus on doing parts of the experiments in order to understand the regulatory relationship of lactic acid bacteria metabolism and raise the productivity of diacetyl by trial and error processes. These take a lot of money and time but in vein. If employing the technology of metabolic engineering, we can make a better mutation strategy for producing diacetyl.
In this study, metabolic flux analysis (MFA) and inverse flux analysis are introduced to analyze the impact of the objective flux when the other flux is slightly perturbed. In addition, we use the quadratic programming optimization to construct the physiological flux distributions of lactic acid bacteria metabolism. Further, we compared the enzyme sets of elementary flux modes with each other to characterize the functional routes and the essential route in lactic acid bacteria metabolism.
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