Antimutagenicity of lactic acid fermentation vegetables extracts against 4-Nitroquinoline N-oxide and Benzo[a]pyrene

• Main Authors: Jui-Ling Hung, 洪瑞琳
• Other Authors: 游若篍
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/90986382119491485130

碩士 === 國立臺灣大學 === 食品科技研究所 === 93 === This study examined the antimutagenic activities of two kinds of lactic acid fermented vegetables Kimchi and Sauerkraut against a direct-mutagen, 4 -nitroquinoline N-oxide (4NQO), and an indirect- mutagen, benzo[a]pyrene (B[a]P), by the Ames test using Salmonella typhimurium TA 98 and TA100. Results indicated that the lactic acid fermented vegetables methanol extracts and water extracts were neither toxic nor mutagenic, whether it existed rat liver extracts (S9 mix) or not. Most Kimchi or Sauerkraut extracts of a concentration of 5 mg/plate showed more than 50% inhibitory effect on 4NQO or B[a]P. This result meant Kimchi or Sauerkraut extracts have antimutagenic activities. The raise of doses of Kimchi and Sauerkraut extracts could increase the antimutagenic activities against 4NQO or B[a]P on S. typhimurium TA 98 and TA100 while extracts were within the rage of concentrations from 0.625 to 5 mg/plate. That showed dose-dependency in the concentration of extracts and the antimutagenic activities of Kimchi and Sauerkraut. According to the preincubation studies, it also discovered that the main mechanism of antimutagenicity against 4NQO is action of desmutagenicity and it against B[a]P is action of blocking effect. It was further studied the effect of antimutagenic activities by adding five strains of bifidobacteria inducing Bifidobacterium bifidum BCRC 14615, B. breve BCRC 11846, B. infantis BCRC 14602, B. longum BCRC 14634 and B. lactis Bb-12. The results showed the antimutagenic activities change by the kind of bifidobacteria and mutagens. When added B. infantis or B. lactis to Sauerkraut, the extracts have measurable effect of antimutagenicity against 4NQO or B[a]P on S. typhimurium TA 98 and TA100 which are higher than the treatment without bifidobacteria. Out of all the groups, the extracts of Kimchi or Sauerkraut adding B. lactis have significantly antimutagenic activities which higher than the treatment without bifidobacteria.