The influence of environmental factors on monacolin K production by Monascus ruber

• Main Authors: Hisn- Yi Tsai, 蔡杏怡
• Other Authors: Long-Shan Lai
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/3pprsh

碩士 === 朝陽科技大學 === 應用化學系碩士班 === 91 === This study is to investigate the effects of culturing factors on monacolin K production by Monascus ruber ATCC 18199. The results of shake-flask experiments showed that the best to 10﹪spores suspension has transferred to production medium after 24h of culture when activity of cellular growth was highest in pre-culture. It was found that pigment production initiated at day 2, followed by monacolin K production at day 6. When the glycerol used was increased to be 176.4 mg/L in the production medium, monacolin K production was increased as 2.7-fold of that in the control group. At the time of the glycerol and peptone the experiment group with G/P ratio 33, the monacolin K increased to 3.52-fold compared with control teams, when the G/P ratio reaches 100 that it is due to inhibition and the G/P ratio is appropriately. In experiments, a simple temperature-shift method proved valuable, where monacolin K was further increased to be 2.18-fold. Meanwhile, it was found that mycelim growth probably favored monacolin K production by the fungus.