Extraction and Purification of Isoflavones from Defatted Soybean Flakes by Using Pressurized Fluids

• Main Authors: Ya-Chuan Cheng, 鄭雅娟
• Other Authors: Chiehming J. Chang
• Format: Others
• Language: zh-TW
• Published: 2001
• Online Access: http://ndltd.ncl.edu.tw/handle/56608671480539927665

碩士 === 國立中興大學 === 化學工程學系 === 89 === This study investigated the effect of extraction conditions on the amount of five isoflavones from defatted soybean flakes (DSF) by using supercritical carbon dioxide extraction (SC-CO2) and pressurized water extraction (PWE), respectively. SC-CO2 extraction conditions were from 2500psig to 4500psig and 313 K to 333 K. PWE extraction conditions were from 300psig to 2200psig and 365 K to 473 K. A RP-HPLC system coupled with an UV detector were utilized to quantify five isoflavones in the extracts. The original content of five isoflavones in DSF powder is 11392μg/g DSF that equals to 1.1 wet%. Furthermore, three adsorbent resins of Amberlite XAD2, XAD7-HP, XAD16-HP were individually used to purify the isoflavones from the extracts. SC-CO2 extraction at 4500psig, 323 K and 5mL/min of preloaded 50g EtOH with 50g DSF powder produced 423μg/g DSF isoflavones. PWE extraction of same feed to solvent ratio DSF powder at 2200psig, 373 K, and 4hr produced 8075μg/g DSF isoflavones, which is equivalent to 70.9% of that by using 80%MeOH Soxhlet extraction. Our experimental results further showed that Amberlite XAD16-HP had the best desorption efficiency (68.3%), when 50%MeOH desorbed solvent was used. The purity of five isoflavones in crystal form was 47.38%.