Neuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum A

Neuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum A

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This project addressed mechanisms of the neuronal DNA damage response after treatment with the model DNA damaging agent ultraviolet light (UV). The thymidine analogue, 5-bromo-2’-deoxyuridine (BrdU) protected against UV-initiated neuronal apoptosis in a concentration-dependent manner (p<0.001). B...

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Bibliographic Details
Main Author: Rajakulendran, Nishani
Other Authors: Laposa, Rebecca
Language:en_ca
Published: 2012
Subjects:
Neuron
DNA repair
BrdU
XPA
CSB
DNA damage
0419
Online Access:http://hdl.handle.net/1807/42406
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spelling ndltd-TORONTO-oai-tspace.library.utoronto.ca-1807-424062013-12-02T03:59:12ZNeuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum ARajakulendran, NishaniNeuronDNA repairBrdUXPACSBDNA damage0419This project addressed mechanisms of the neuronal DNA damage response after treatment with the model DNA damaging agent ultraviolet light (UV). The thymidine analogue, 5-bromo-2’-deoxyuridine (BrdU) protected against UV-initiated neuronal apoptosis in a concentration-dependent manner (p<0.001). BrdU did not protect proliferating mouse embryonic fibroblasts from UV-induced apoptosis. We assessed whether the mechanism of BrdU neuroprotection was through a modification in the neuronal DNA damage response. BrdU neuroprotection was independent of BrdU incorporation into DNA, neuronal DNA repair, p53 activation or cell cycle re-entry, a neuronal DNA damage response. Neurons deficient in Cockayne Syndrome B (CSB) or Xeroderma Pigmentosum A (XPA) were paradoxically resistant to UV-initiated apoptosis. Therefore, CSB and XPA play essential roles in the neuronal DNA damage response.Laposa, Rebecca2012-112013-11-15T17:59:06ZWITHHELD_ONE_YEAR2013-11-15T17:59:06Z2013-11-15Thesishttp://hdl.handle.net/1807/42406en_ca
collection NDLTD
language en_ca
sources NDLTD
topic Neuron
DNA repair
BrdU
XPA
CSB
DNA damage
0419
spellingShingle Neuron
DNA repair
BrdU
XPA
CSB
DNA damage
0419
Rajakulendran, Nishani
Neuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum A
description This project addressed mechanisms of the neuronal DNA damage response after treatment with the model DNA damaging agent ultraviolet light (UV). The thymidine analogue, 5-bromo-2’-deoxyuridine (BrdU) protected against UV-initiated neuronal apoptosis in a concentration-dependent manner (p<0.001). BrdU did not protect proliferating mouse embryonic fibroblasts from UV-induced apoptosis. We assessed whether the mechanism of BrdU neuroprotection was through a modification in the neuronal DNA damage response. BrdU neuroprotection was independent of BrdU incorporation into DNA, neuronal DNA repair, p53 activation or cell cycle re-entry, a neuronal DNA damage response. Neurons deficient in Cockayne Syndrome B (CSB) or Xeroderma Pigmentosum A (XPA) were paradoxically resistant to UV-initiated apoptosis. Therefore, CSB and XPA play essential roles in the neuronal DNA damage response.
author2 Laposa, Rebecca
author_facet Laposa, Rebecca
Rajakulendran, Nishani
author Rajakulendran, Nishani
author_sort Rajakulendran, Nishani
title Neuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum A
title_short Neuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum A
title_full Neuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum A
title_fullStr Neuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum A
title_full_unstemmed Neuronal UV-Initiated Apoptosis is Prevented By 5-Bromo-2’-Deoxyuridine (BrdU) Or A Deficiency in Cockayne Syndrome B Or Xeroderma Pigmentosum A
title_sort neuronal uv-initiated apoptosis is prevented by 5-bromo-2’-deoxyuridine (brdu) or a deficiency in cockayne syndrome b or xeroderma pigmentosum a
publishDate 2012
url http://hdl.handle.net/1807/42406
work_keys_str_mv AT rajakulendrannishani neuronaluvinitiatedapoptosisispreventedby5bromo2deoxyuridinebrduoradeficiencyincockaynesyndromeborxerodermapigmentosuma
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