Local hydrogen deuterium exchange mass spectrometry: from pressurized online digestion to pepsin proteolysis.

• Online Access: http://hdl.handle.net/2047/d20003044

Higher-order structure of a protein is important for proper biological function. Current analytical and structural methods provide comprehensive understanding of the chemical, physical, and biological characteristics of biologics, yet detecting a change in dynamics and conformation can be a challenging task. Hydrogen deuterium exchange mass spectrometry (HDX MS) can be used to probe conformational changes of proteins. It is particularly useful to detect subtle changes in conformation at the peptide level. It is critical to generate many different kinds of overlapping peptides that are reproducible, so they can be used to enhance sequence coverage and spatial resolution.