Role of residues on the proximal side of the heme in catalase HPII of Escherichia coli
Through site-directed mutagenesism the roles of the amino acids H392, H395, D197, Q419 in heme conversion were studied. H392 mutants which disable the His-Tyr bond contain only heme b as their prosthetic group, and lose specific activity, thermostability and sensitivity to the inhibitors NaCN, NaN3...
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| Language: | en_US |
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2007
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| Online Access: | http://hdl.handle.net/1993/2389 |